eprintid: 14373 rev_number: 14 eprint_status: archive userid: 5 dir: disk0/00/01/43/73 datestamp: 2017-02-06 09:52:48 lastmod: 2021-08-27 17:28:32 status_changed: 2017-02-06 09:52:48 type: article metadata_visibility: show creators_name: Furlan, V. creators_name: Maus, V. creators_name: Batista, I. creators_name: Bandarra, N.A. creators_id: 8780 creators_orcid: 0000-0002-7385-4723 title: Production of docosahexaenoic acid by Aurantiochytrium sp. ATCC PRA-276 ispublished: pub divisions: prog_esm keywords: Carbon source; Docosahexaenoic acid; Nitrogen source; Polyunsaturated fatty acids; Thraustochytrids abstract: The high costs and environmental concerns associated with using marine resources as sources of oils rich in polyunsaturated fatty acids have prompted searches for alternative sources of such oils. Some microorganisms, among them members of the genus Aurantiochytrium, can synthesize large amounts of these biocompounds. However, various parameters that affect the polyunsaturated fatty acids production of these organisms, such as the carbon and nitrogen sources supplied during their cultivation, require further elucidation. The objective of this investigation was to study the effect of different concentrations of carbon and total nitrogen on the production of polyunsaturated fatty acids, particularly docosahexaenoic acid, by Aurantiochytrium sp. ATCC PRA-276. We performed batch system experiments using an initial glucose concentration of 30 g/L and three different concentrations of total nitrogen, including 3.0, 0.44, and 0.22 g/L, and fed-batch system experiments in which 0.14 g/L of glucose and 0.0014 g/L of total nitrogen were supplied hourly. To assess the effects of these different treatments, we determined the biomass, glucose, total nitrogen and polyunsaturated fatty acids concentration. The maximum cell concentration (23.9 g/L) was obtained after 96 h of cultivation in the batch system using initial concentrations of 0.22 g/L total nitrogen and 30 g/L glucose. Under these conditions, we observed the highest level of polyunsaturated fatty acids production (3.6 g/L), with docosahexaenoic acid and docosapentaenoic acid ω6 concentrations reaching 2.54 and 0.80 g/L, respectively. date: 2017-04-01 date_type: published publisher: Sociedade Brasileira de Microbiologia id_number: 10.1016/j.bjm.2017.01.001 creators_browse_id: 3138 full_text_status: public publication: Brazilian Journal of Microbiology volume: 48 number: 2 pagerange: 359-365 refereed: TRUE issn: 1678-4405 coversheets_dirty: FALSE fp7_project: no fp7_type: info:eu-repo/semantics/article citation: Furlan, V., Maus, V. ORCID: https://orcid.org/0000-0002-7385-4723 , Batista, I., & Bandarra, N.A. (2017). Production of docosahexaenoic acid by Aurantiochytrium sp. ATCC PRA-276. Brazilian Journal of Microbiology 48 (2) 359-365. 10.1016/j.bjm.2017.01.001 . document_url: https://pure.iiasa.ac.at/id/eprint/14373/1/1-s2.0-S151783821730028X-main.pdf