Production of docosahexaenoic acid by Aurantiochytrium sp. ATCC PRA-276

14373 14 75936 5 211980 document 75936 1-s2.0-S151783821730028X-main.pdf application/pdf b3ca4114cde1b191adba97db47d2f972 MD5 1010461 2017-03-23 09:37:38 https://pure.iiasa.ac.at/id/eprint/14373/1/1-s2.0-S151783821730028X-main.pdf 14373 1 1 application/pdf text en public cc_by_nc_nd

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published 78747 1 217504 document 78747 indexcodes.txt text/x-c af03338a22648d8bba19c1b18622a652 MD5 9842 2017-08-05 12:53:15 https://pure.iiasa.ac.at/id/eprint/14373/2/indexcodes.txt 14373 2 2 text/x-c other Generate index codes conversion from text to indexcodes en public

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http://eprints.org/relation/isVersionOf https://pure.iiasa.ac.at/id/document/75936 http://eprints.org/relation/isVolatileVersionOf https://pure.iiasa.ac.at/id/document/75936 http://eprints.org/relation/issmallThumbnailVersionOf https://pure.iiasa.ac.at/id/document/75936 archive 5 disk0/00/01/43/73 2017-02-06 09:52:48 2021-08-27 17:28:32 2017-02-06 09:52:48 article show Furlan V. Maus V. 8780 0000-0002-7385-4723 Batista I. Bandarra N.A. Production of docosahexaenoic acid by Aurantiochytrium sp. ATCC PRA-276 pub prog_esm Carbon source; Docosahexaenoic acid; Nitrogen source; Polyunsaturated fatty acids; Thraustochytrids The high costs and environmental concerns associated with using marine resources as sources of oils rich in polyunsaturated fatty acids have prompted searches for alternative sources of such oils. Some microorganisms, among them members of the genus Aurantiochytrium, can synthesize large amounts of these biocompounds. However, various parameters that affect the polyunsaturated fatty acids production of these organisms, such as the carbon and nitrogen sources supplied during their cultivation, require further elucidation. The objective of this investigation was to study the effect of different concentrations of carbon and total nitrogen on the production of polyunsaturated fatty acids, particularly docosahexaenoic acid, by Aurantiochytrium sp. ATCC PRA-276. We performed batch system experiments using an initial glucose concentration of 30 g/L and three different concentrations of total nitrogen, including 3.0, 0.44, and 0.22 g/L, and fed-batch system experiments in which 0.14 g/L of glucose and 0.0014 g/L of total nitrogen were supplied hourly. To assess the effects of these different treatments, we determined the biomass, glucose, total nitrogen and polyunsaturated fatty acids concentration. The maximum cell concentration (23.9 g/L) was obtained after 96 h of cultivation in the batch system using initial concentrations of 0.22 g/L total nitrogen and 30 g/L glucose. Under these conditions, we observed the highest level of polyunsaturated fatty acids production (3.6 g/L), with docosahexaenoic acid and docosapentaenoic acid ω6 concentrations reaching 2.54 and 0.80 g/L, respectively. 2017-04-01 published Sociedade Brasileira de Microbiologia 10.1016/j.bjm.2017.01.001 3138 public Brazilian Journal of Microbiology 48 2 359-365 TRUE 1678-4405 FALSE no info:eu-repo/semantics/article