Soils as key component of terrestrial ecosystems are under increasing pressures. As an advance to current static assessments, we present a dynamic soil functions assessment (SFA) to evaluate the current and future state of soils regarding their nutrient storage, water regulation, productivity, habitat and carbon sequestration functions for the case-study region in the Lower Austrian Mostviertel. Carbon response functions simulating the development of regional soil organic carbon (SOC) stocks until 2100 are used to couple established indicator-based SFA methodology with two climate and three land use scenarios, i.e. land sparing (LSP), land sharing (LSH), and balanced land use (LBA). Results reveal a dominant impact of land use scenarios on soil functions compared to the impact from climate scenarios and highlight the close link between SOC development and the quality of investigated soil functions, i.e. soil functionality. The soil habitat and soil carbon sequestration functions on investigated agricultural land are positively affected by maintenance of grassland under LSH (20 of the case-study region), where SOC stocks show a steady and continuous increase. By 2100 however, total regional SOC stocks are higher under LSP compared to LSH or LBA, due to extensive afforestation. The presented approach may improve integrative decision-making in land use planning processes. It bridges superordinate goals of sustainable development, such as climate change mitigation, with land use actions taken at local or regional scales. The dynamic SFA broadens the debate on LSH and LSP and can reduce trade-offs between soil functions through land use planning processes.