Hemidactylus mabouia (Moreau de Jonnès, 1818) is recorded for the first time from the remote island of Saint Helena, based on a specimen observed by one of the coauthors and several further photographic records verified from the citizen science platform iNaturalist. Comparison is made with the well-established and widespread Hemidactylus frenatus Duméril & Bibron, 1836 which was hitherto the only established gecko known from Saint Helena. Notes on reptiles on the neighbouring Ascension Island, especially its geckos, are provided.